Gradient plate technique for study of bacterial resistance

How to prepare and use gradient plates. David Perkins

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How to prepare and use gradient plates. David Perkins Background Petri plates with a continuous gradient of concentrations from one side to the other were described by Szybalski and Bryson (1952). It was used by them to select bacterial mutants that had acquired increased resistance to antibiotics.

How to prepare and use gradient plates. David Perkins

Gradient plate method to induce Streptococcus pyogenes ...

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The aim of this study was to compare in-vitro development of resistance of streptococci to β -lactams and erythromycin by serial passages in subinhibitory concentrations of antibiotics by a gradient plate method. Material and methods. Three clinical strains of S. pyogenes were tested. Two strains were susceptible to erythromycin (MIC 0.015 mg ...

Gradient plate method to induce Streptococcus pyogenes ...

The gradient plate technique as a means of studying the ...

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Overall, it is suggested that the gradient plate technique, in combination with image analysis, could be useful in determining combinations of di•erent environmental factors which are e•ective in preventing the recovery of heat injured bacterial cells. Keywords Combined e•ect of pH and NaCl, culture age, heat resistance, image analysis.

The gradient plate technique as a means of studying the ...

Development of bacterial resistance to several biocides ...

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The aims of this study were to investigate the development of bacterial resistance to eugenol, thymol, trichlorocarbanalide (TCC), didecyldimethylammonium chloride (DDDMAC) and C10-16 ...

Development of bacterial resistance to several biocides ...

Use of Gradient Plates To Study Combined Effects of ...

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5/17/2004 · The effect of temperature, pH, and sodium chloride concentration on the growth of the Ascomycetes fungus Monascus ruber van Tieghem, the main spoilage microorganism during storage of table olives, was studied by using the gradient plate technique. Gradients of NaCl (3 to 9%, wt/vol) at right angles to gradients of pH (2 to 6.8) were prepared for the plates, which were incubated at 25, 30, and ...

Use of Gradient Plates To Study Combined Effects of ...

modified population analysis profile (PAP) method to ...

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The gradient plate technique as a means of studying the recovery of heat-injured Brochothrix thermosphacta. ... were used required to inhibit the growth of Listeria mono- to study bacterial heat resistance. cytogenes. In a subsequent study, image analysis was used to map bacterial growth by placing agar Thermal inactivation studies plates ...

modified population analysis profile (PAP) method to ...

(PDF) The gradient plate technique as a means of studying ...

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Amodification of the original gradient-plate method has been devised and found to improve the utility and accuracy of this technique when TABLE 1. Determination ofMIC ofamphotericin Ba Conventional Trilevel Trial gradient-plate gradient-plate methodb method' 1 13.9 13.9 2 7.0 14.7 3 8.3 11.1 4 18.2 14.0 5 10.9 12.1 a Values are expressed as ...

(PDF) The gradient plate technique as a means of studying ...

Improved Agar Gradient-Plate Technique

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Also of interest is the greater resistance of culture nio. 2 to National Formulary tyrothricin when ... of the assay, suggest that the gradient plate technique ... Agradient plate technique for the study of bacterial resistance. Science 116:46-48.

Improved Agar Gradient-Plate Technique

Gradient Plate Bioassay Application Dentifrices

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This study aimed to determine the degree of susceptibility of several industrial bacterial isolates tolerant to para-chloro-meta-xylenol, triclosan, three other commonly used biocides, and to a range of clinically significant antibiotics.Minimum inhibitory concentrations (MICs) of benzalkonium chloride, chlorhexidine and phenol were determined and compared with those of standard strains.

Gradient Plate Bioassay Application Dentifrices

Chloroxylenol- and triclosan-tolerant bacteria from ...

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Portaels, F.: Unclassified mycobacterial strains susceptible to dapsone isolated from the environment in Central Africa. Int. J. Leprosy 48 (1980) 330-331 8. Szybalski, W.: Microbial selection I. Gradient plate technique for study of bacterial resistance. Science 116 (1952) 46-48 9.

Chloroxylenol- and triclosan-tolerant bacteria from ...

On the possibility of cross-resistance between rifampicin ...

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Discrete colonies were then spread onto a gradient plate with higher antibiotic concentrations; the boundary line increased significantly, and gene mutations conferring resistance were identified. This new method enables the rapid identification of resistant strains in the bacterial population. Use of …

On the possibility of cross-resistance between rifampicin ...

SCIENCE CHINA Life Sciences - link.springer.com

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The gradient plate technique as a means of studying the recovery of heat‐injured Brochothrix thermosphacta ... This study used agar gradient plates, in combination with image analysis, to study ...

SCIENCE CHINA Life Sciences - link.springer.com

The gradient plate technique as a means of studying the ...

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Bacterial Sensitivity. Determination of comparative resistance: The gradient plate technique was used in the determination of comparative resistance. A bacterial suspension streaked over the agar surface can be a direct measure of the inhibitory concentration by measuring the length of the streak. Size of streaks relative to antibiotic resistance

The gradient plate technique as a means of studying the ...

Genetic Studies of Microbial Cross Resistance to Toxic ...

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On this agar plate, a bacterial isolate is tested for resistance to each of twelve different antibiotics. The clear zones around each disc are the zones of inhibition that indicate the extent of the test organism’s inability to survive in the presence of the test antibiotic.

Genetic Studies of Microbial Cross Resistance to Toxic ...

Examples of Antibiotic Sensitivity Testing Methods ...

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Cross Resistance of Bacillus megaterium to Forty-Four Antimicrobial Drugs' WACLAW SZYBALSKI ... This type of study affords opportunity for the selec-tion of some mutants which are not related by cross ... Bacterial stocks were grown in small (1-oz) screw-cap bottles filled with double strength

Examples of Antibiotic Sensitivity Testing Methods ...

Genetic Studies on Microbial Cross Resistance Agents ...

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E. coli Antibiotic Resistance 4 Escherichia coli and Antibiotic Resistance to Tetracycline Antibiotics Bacterial antibiotic resistance The phenomenon of bacterial drug resistance was first documented in 1951 (1). Interest in bacterial antibiotic resistance continues to increase as some antibiotics are less

Genetic Studies on Microbial Cross Resistance Agents ...

Escherichia coli and Antibiotic Resistance to Tetracycline ...

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This system is a specialized dispenser, which distributes a single FabI mutation, multiple mechanisms may be responsible for liquid samples in an Archimedes spiral onto the surface of a rotating agar triclosan resistance.17 For instance, bacterial efflux pumps (RND plate from the centre to the edge, such that an ∼1000-fold concentration ...

Escherichia coli and Antibiotic Resistance to Tetracycline ...

Selection for high-level resistance by chronic triclosan ...

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The gradient plate technique as a means of studying the recovery of heat‐injured Brochothrix thermosphacta. ... This study used agar gradient plates, ... broth at 25 °C were heat‐treated at 50 °C to measure thermal resistance.

Selection for high-level resistance by chronic triclosan ...

The gradient plate technique as a means of studying the ...

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Antimicrobial resistance (AMR or AR) is the ability of a microbe to resist the effects of medication that once could successfully treat the microbe. The term antibiotic resistance (AR or ABR) is a subset of AMR, as it applies only to bacteria becoming resistant to antibiotics. Resistant microbes are more difficult to treat, requiring alternative medications or higher doses of antimicrobials.

The gradient plate technique as a means of studying the ...

Antimicrobial resistance - Wikipedia

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Full text of "Sporogenesis in bacteria : a study of techniques" See other formats SPOROGENESIS IN BaCTERIa A STUDY OF TECHNIQUES by RAMESH LALMWBOTRA Graduate Bihar Veterinary College, Patna, 1944 Post-Graduate (Bact.), Indian Veterinary Research Institute, U. P., 1996 A MASTER^ THESIS submitted in partial fulfillment of the requirement* for the degree MASTER OF …

Antimicrobial resistance - Wikipedia

Full text of "Sporogenesis in bacteria : a study of ...

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3/1/2012 · Target Audience: The target audience for Mayo Clinic Proceedings is primarily internal medicine physicians and other clinicians who wish to advance their current knowledge of clinical medicine; and who wish to stay abreast of advances in medical research. Statement of Need: General internists and primary care providers must maintain an extensive knowledge base on a wide variety of …

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